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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC, ELISA | M | Affinity Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN633 |
| Replaces | 04-1053 |
| Description | Anti-a-Synuclein, clone 10D2 Antibody |
| Alternate Names |
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| Background Information | α-Synuclein, also known as Non-A beta component of AD amyloid or Non-A4 component of amyloid, or NACP, and encoded by the gene SNCA/NACP/PARK1, is a protein originally identified as non-Abeta component protein (NACP) in the amyloid enriched fraction isolated from Alzheimer’s brain tissue. α-Synuclein is expressed in neural tissue and non-neural tissues too. In neurons, α-Synuclein is localized to the nucleus, presynaptic termini and mitochondria of certain brain regions. α-Synuclein is a molecular chaperone that binds various proteins and compounds including synaptobrevin-2 protein and membrane phospholipids and is involved in vesicle trafficking and membrane transport within neurons. Natural, non-mutated α-Synuclein has autoproteolytic activity and naturally forms stable alpha helical tetramers. However, mutated α-Synuclein can aggregate to form insoluble pathological complexes such as Lewy Bodies inside neurons. Lewy Bodies also contain ubiquitin and it is thought that they are evidence of disrupted proteasome function with regards to α-synuclein but research is still continuing. This antibody can be used as a sandwich ELISA pair with Cat. No. MABN389, Anti-Aggregated a-Synuclein, clone 5G4. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-a-Synuclein, clone 10D2 Antibody is an antibody against a-Synuclein for use in western blotting, IHC & ELISA. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot detected aggregated a-Synuclein in human brain tissues. ELISA Analysis: A representative lot from an independent laboratory detected aggregated a-Synuclein in a sandwich ELISA with Cat. No. MABN389, Anti-Aggregated a-Synuclein, clone 5G4 (Kovacs, G. G., et al. (2012). Acta Neuropathol. 123(1):37-50.). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human Synuclein. |
| Clone | 10D2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~15 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human brain tissue lysate. Western Blotting Analysis: 1.5 µg/mL of this antibody detected a-Synuclein in 10 µg of human brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |