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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB, IF, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN616 |
| Description | Anti-Potassium Channel Kv1.1 Antibody, clone K36/15 |
| Alternate Names |
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| Background Information | Potassium voltage-gated channel subfamily A member 1, better known as Potassium Channel Kv1.1, or Kcna1 belongs to the Shaker potassium channel group responsible for mediating ion permeability of excitable membranes. Conformational changes as a result of voltage changes across the cell membrane varies the permeability of this multi-pass transmembrane channel to potassium. Mutations of the Shaker potassium channel gene in Drosophila and the KCNA1 gene for its mammalian orthologue, Kv1.1, reduce the probability of action potential failure thereby causing hyperexcitability near axon branch points and nerve terminals, thereby leading to uncontrolled movements and recapitulating the episodic ataxia-1 (EA1) symptoms in human patients. Further, the axonal Kv1 channel activity may regulate synaptic transmission by modulating the action potential waveform, duration, firing pattern, and spread of depolarization into axonal branches. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Potassium Channel Kv1.1 Antibody, clone K36/15 is a highly specific mouse monoclonal antibody, that targets Kv1.1 & has been tested in Western blotting, Immunofluorescence & IHC. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 4 µg/mL from a representative lot detected Potassium Channel Kv1.1 in 10 µg of rat brain membrane tissue lysate. Immunofluoresense Analysis: A representative lot from an independent laboratory detected Potassium Channel Kv1.1 in Kv1.1 transfected COS-1 cells (Tiffany, A. M., et al. (2000). J Cell Biol. 148(1):147-158.). Immunhistochemistry Analysis: A representative lot from an independent laboratory detected Potassium Channel Kv1.1 in Kv1.1 transfected COS-1 cells (Wenzel. H. J., et al (2007). Epilepsia. 48(11):2023-2046.). Immunohistochemistry Analysis: A representative lot from an independent laboratory detect Potassium Channel Kv1.1 in wild type mouse hippocampus tissue, and demonstrated a loss of signal in Potassium Channel Kv1.2 knock out mouse hippocampus tissue (Prof. J. Trimmer, University of California, Davis.). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to 18 amino acids from the extracellular domain of rat Potassium Channel Kv1.1. |
| Clone | K36/15 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with Mouse and Rat. Other homologies: Human (94% sequence homology). |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~65 kDa observed. The calculated molecular weight of this protein is 56 kDa. However, it can run higher due to glycosylation (Deal, K. K., et al. (1994). J Neurosci. 14(3 Pt 2):1666-1676.). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected Potassium Channel Kv1.1 in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |