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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN497 |
| Description | Anti-PSD93 Antibody, clone N18/30 |
| Alternate Names |
|
| Background Information | As a member of the membrane-associated guanylate kinase (MAGUK) family, PSD93 (also known as Chapsyn-110) binds directly to the N-methyl-D-aspartate (NMDA) receptor and the Shaker K+ channel subunits. It shares 70-80% homology with PSD-95 (SAP90), and SAP97. Like PSD-95, PSD93 plays a key role in postsynaptic targeting. Aside from binding to glutamate receptors, the MAGUK family of proteins also interacts with cell surface structures, like cell adhesion molecules, ion channels, cytoskeletal elements, and signal transduction molecules. Improper PSD93 signaling has been implicated in certain disease states, such as schizophrenia. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect PSD93 using this Anti-PSD93 Antibody, clone N18/30 validated for use in Western Blotting & IHC. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1 µg/mL from a representative lot detected PSD93 in 10 µg of human brain tissue lysate. Immunohistochemistry Analysis: A representative lot detected PSD93 in rat brain tissue (Prof. J. Trimmer, University of California, Davis). Western Blot Analyis: A representative lot detected PSD93 in WT, but not in PSD93 knockout mice. Western Blot Analysis: A representative lot detected PSD93 in CA1 tissue lysates from C57BL/6 mice (Jeffrey, R. A., et al. (2009). J Neurosci. 29(50):15613-15620.). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to rat PSD93. |
| Clone | N18/30 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Mouse, Human, and Rat. This antibody does not demonstrate cross reactivity with other MAGUK family members, such as PSD-95, SAP97, and SAP102 expressed in transfected cells. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~110 kDa observed. The calculated molecular weight of PSD93 is 95 kDa; however, this protein is highly phosphorylated and is observed at ~110 kDa. Multiple isoforms between 68-98 kDa are known to exist. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PSD93 in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |