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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN483 |
| Description | Anti-IRAP Antibody, Clone 3E1 |
| Alternate Names |
|
| Background Information | IRAP, also known as Leucyl-cystinyl aminopeptidase, Cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase (IRAP), Oxytocinase (OTase), or Placental leucine aminopeptidase (P-LAP) and encoded by the human gene name IRAP/OTASE, is an important aminopeptidase that plays an important role in maintaining homeostasis during pregnancy. IRAP degrades peptide hormones including oxytocin, vasopressin, and angiotensin III; it also appears to play a role in degrading neuronal peptides in the brain such as Met-enkephalin and dynorphin. IRAP also binds angiotensin IV and appears to be the receptor for angiotensin IV in the brain. IRAP is a membrane bound aminopeptidase and is often found within membrane vesicles that together with other proteins like GLUT4 translocates to the plasma membrane in response to insulin or oxytocin. A cleaved fragment of IRAP, the Leucyl-cystinyl aminopeptidase, is found in the blood during pregnancy, low in the first trimester but rising rapidly until birth, whereby it decreases rapidly, thus the secreted form is often used as a pregnancy marker. IRAP is highly expressed in placenta, heart, kidney and small intestine. IRAP is detected at lower levels in neuronal cells in the brain, in skeletal muscle, spleen, liver, testes and colon. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-IRAP, Clone 3E1 is an antibody against IRAP for use in WB, ICC. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected IRAP in 3T3-L1 cells treated with or without insulin (Garza, L.A., et al. (2000). JBC. 275(4):2560-2567). Immunocytochemistry Analysis: A representative lot detected IRAP in 3T3-L1 cells treated with or without insulin (Garza, L.A., et al. (2000). JBC. 275(4):2560-2567). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the N-terminus of human IRAP. |
| Epitope | N-terminus |
| Clone | 3E1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~150 kDa observed. The calculated molecular weight of this protein is 117 kDa, but can be observed at ~150 kDa due to high glycosylation. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in insulin treated NIH-3T3 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected IRAP in 10 µg of insulin treated NIH-3T3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |