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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN466 |
| Description | Anti-Ankyrin-G Antibody, clone N106/36 |
| Alternate Names |
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| Background Information | Ankyrins are a family of spectrin-binding proteins that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins which include channels such as the anion exchanger, voltage-dependent sodium channel, and the Na/K ATPase, as well as neuronal cell adhesion molecules related to L1. In the nervous system, three different ankyrins are known to exist; ankyrinB, ankyrinG, and ankyrinR. All three ankyrins contain an N-terminal membrane binding domain, an internal domain capable of binding spectrin, and a C-terminal regulatory domain which is the target of alternative splicing. AnkyrinB, the predominant neuronal ankyrin includes two isoforms of ~220 kDa and ~440 kDa. The ~220 kDa alternatively spliced isoform is the major form present in the adult brain, while the ~440 kDa form is preferentially expressed in the developing neonatal brain. Three forms of ankyrinG (~190 kDa, ~270 kDa, ~480 kDa) are localized in axonal initial segments and the nodes of Ranvier of myelinated axons where fluxes of action potential occur and ion channels are enriched. Ankyrins comprise ~0.5%-1% of the total membrane protein in the adult vertebrate brain. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG2aκ in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Ankyrin-G Antibody, clone N106/36 is a highly specific mouse monoclonal antibody & that targets Ankyrin & has been tested in IHC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Ankyrin-G in rat cerebral cortex tissue. Immunohistochemistry Analysis: A representative lot detected Ankyrin-G in rat optic nerve tissue. Immunofluorescence Analysis: A representative lot detected Ankyrin-G in adult rat cortex tissue. Immunofluorescence Analysis: A representative lot from an independent laboratory detected Ankyrin-G in a rat model of TLE mEC layer II neurons (Hargus, N. J., et al. (2011). Neurobiol Dis. 41(2):361-376.) |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human Ankyrin-G. |
| Clone | N106/36 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with Rat. Other homologies: Human (92% sequence homology), and Mouse (87% sequence homology). |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | 480 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in rat hippocampus tissue. Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Ankyrin-G in rat hippocampus tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |