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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN42 |
| Description | Anti-Neuregulin-1 Antibody, clone D11 |
| Alternate Names |
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| Background Information | The group of Neuregulin-1 proteins are cell-cell signaling molecules and are ligands for receptor tyrosine kinases of the ErbB/HER subfamily. Type III NRG1 proteins (also referred to as CRD-NRG1 proteins) play a critical role in neural development, including myelination, motor and sensory neuron survival, and neuromuscular synapse development (Falls, 2003). Defects in animals lacking type III NRGs include retraction of nerve terminals from newly formed synapses, absence of Schwann cells from peripheral nerves, and loss of motor and sensory neurons (Wolpowitz et al., 2000). Available evidence indicates that most Type III NRG1 proteins are synthesized as transmembrane proteins with 2 TM domains, and that proteolytic processing produces a bioactive transmembrane form (NTFm) which serves as a juxtacrine signal (Wang, 2001; Falls, 2003). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Neuregulin-1 using this Anti-Neuregulin-1 Antibody, clone D11 validated for use in WB & IC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A previous lot of this antibody was used successfully by an independent laboratory in IC. (Pirotte, D., et al.) |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant protein corresponding to mouse Neuregulin-1 at the extracellular domain. |
| Epitope | Extracellular domain |
| Clone | D11 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes Neuregulin-1 at the extracellular domain. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G |
| UniProt Number |
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| Molecular Weight | ~29 kDa observed. This target has been shown to undergo differential splicing during development into ~30 different isoforms. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse brain microsomal tissue lysate. Western Blot Analysis: 0.25 µg/mL of this antibody detected Neuregulin-1 on 10 µg of mouse brain microsomal tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |