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Millipore Anti-App-C99 Antibody, Clone Mc99(70–80) Is A Highly Specific Mouse Monoclonal Antibody, That Targets App & Has Been Tested In Western Blotting, Ip, Ihc & If. - Mill (Additional S&H or Hazmat Fees May Apply)
Amyloid Precursor Protein (APP) is expressed in the brain, kidney, heart, and spleen of fetal tissues; it is induced during neuronal differentiation. The most-substantiated role for APP is in synaptic formation and repair; its expression is upregulated during neuronal differentiation and after neural injury. In adult brain, highest expression of APP is found in the frontal lobe of the cortex. Moderate expression is observed in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimers disease (AD). UP on intracerebroventricular injection of this antibody, A-beta levels significantly decreased in the brain of APPPS1 mouse model of AD. A related antibody, Cat. No. MABN381, Anti-APP-C99, clone mC99(1-7) was raised against a slightly different region of the same APP-C99 protein.
Product Information
Format
Ascites
Presentation
Mouse monoclonal IgGκ ascites
Quality Level
MQ100
Applications
Application
Anti-APP-C99 Antibody, clone mC99(70–80) is a highly specific mouse monoclonal antibody, that targets APP & has been tested in western blotting, IP, IHC & IF.
Key Applications
Western Blotting
Immunoprecipitation
Immunohistochemistry
Immunofluorescence
Application Notes
Immunoprecipitation Analysis: A representative lot immunoprecipitated APP-C99 in HEK APPSwe cell lysate (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).
Immunohistochemistry Analysis: A representative lot detected APP-C99 in APPS1 mice and in human Alzheimer brain tissues (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).
Immunofluoresence Analysis: A representative lot detected APP-C99 in HEK APPSwe cells (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).
Biological Information
Immunogen
Recombinant protein corresponding to Human APP-C99.
Clone
mC99(70-80)
Host
Mouse
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000475
Gene Symbol
APP
A4
AD1
Purification Method
Unpurified
UniProt Number
P05067
Molecular Weight
~95 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in DAPT treated HEK293 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected APP-C99 in 10 µg of DAPT treated HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
