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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN361 |
| Description | Anti-VAPA Antibody, clone 7E10.1 |
| Alternate Names |
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| Background Information | Vesicle-associated membrane protein-associated protein A (UniProt Q9P0L0; also known as 33 kDa VAMP-associated protein, hVAP-33, VAMP-A, VAMP-associated protein A, VAP-33, VAP-A) is encoded by the VAPA (also known as VAP33) gene (Gene ID 9218) in human. VAMP-associated proteins (VAPs) are type IV membrane proteins that are well conserved among species. There exist three human VAPs encoded by two genes, with VAPA encoding VAP-A and VAPB encoding VAP-B and VAP-C. VAPs generally localize at the endoplasmic reticulum (ER), although they are also reported to localize at other subcellular organelles in some species and cell types. VAP-A contains a major sperm protein (MSP) domain at the N-terminal, followed by a coiled-coil domain, and a transmembrane (TM) domain. VAPs were shown to have important roles in non-vesicular lipid transport, lipid metabolism, the regulation of ER structure, and the unfolded protein response through MSP domain-mediated interaction with FFAT motifs. Oxysterol-binding protein (OSBP) is a cytosolic receptor of cholesterol and oxysterols. OSBP is recruited to the ER by binding to the MSP domain of VAP-A, a process essential for the stimulation of sphingomyelin synthesis by 25-hydroxycholesterol. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-VAPA Antibody, clone 7E10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of VAPA. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.2 µg/mL from a representative lot detected VAPA in 10 µg of mouse brain tissue lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected VAPA in human cerebellum and human testis tissue. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the C-terminal half of human VAPA. |
| Epitope | C-terminal half |
| Clone | 7E10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Cross-reactivity to human VAPA spliced isoform 2 is highly expected, but not confirmed. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~30 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected VAPA in 10 µg of SH-SY5Y cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |