Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IH(P), ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1838 |
| Description | Anti-AGE Antibody, Methylglyoxal Antibody, clone 3D11 |
| Alternate Names |
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| Background Information | Chronic accumulation of Advanced Glycation End products (AGEs) is a common feature during aging, hyperlipidemia, and diabetes and affects function of proteins, lipids, and DNA. Although formation of AGE products is considered to be predominantly an endogenous process, certain external factors, such as some dietary products and smoking can increase the level of AGE in tissues. Under certain conditions glucose may form covalent adducts with the plasma proteins through the process of glycation. Glycation is a non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of proteins. The term AGE often refers to non-reactive terminal products, such as N- carboxymethyllysine (CML) as well as reactive precursors, such as methylglyoxal (MG), which is generated through a non-oxidative process from triose phosphates during anaerobic glycolysis. MG reacts with arginine, lysine, and cysteine residues in proteins to form AGE products. Excessive glycation can disrupt protein function by altering enzyme activity, receptor function, and molecular conformation. Protein glycation and subsequent formation of AGE products contributes to the pathogenesis of diabetes-related complications, such as retinopathy, nephropathy, and neuropathy. AGE products interact with a variety of cell-surface AGE-binding receptors (RAGE) that results in their endocytosis and degradation. It can also generate pro-oxidant and pro-inflammatory events in cells. AGE products could also contribute to the development or worsening of many degenerative diseases, including Alzheimers disease. (Ref.: Cai,W., et al. (2002). Mol. Med 8 (7): 337-346). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-AGE, Methylglyoxal, clone 3D11, Cat. No. MABN1838, is a highly specific mouse monoclonal antibody that targets Methylglyoxal modified proteins and has been tested in ELISA and Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | ELISA Analysis: A representative lot detected AGE, Methylglyoxal in ELISA applications (Vlassara, H., et. al. (2002). Proc Natl Acad Sci USA. 99(24):15596-601; Cai, W., et. al. (2008). Am J Pathol. 173(2):327-36; Cai, W., et. al. (2012). Proc Natl Acad Sci USA. 109(39):15888-15893). |
| Biological Information | |
|---|---|
| Immunogen | Methylglyoxal-cojugated Ovalbumin. |
| Clone | 3D11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3D11 specifically reacts with methylglyoxal modified proteins. It does not react with CML, CEL, or other AGE epitopes. |
| Isotype | IgG2aλ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with Human and Rat. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein G purified |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in mouse kidney tissue. Immunohistochemistry Analysis: A 1:3,000 dilution of this antibody detected AGE, Methylglyoxal in kidney tissue from mice fed high AGE diets. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |