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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ELISA, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1796 |
| Description | Anti-LRP1 Antibody, 85 kDa subunit Antibody, clone 6F8 |
| Alternate Names |
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| Background Information | Prolow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APER, Apolipoprotein E receptor, CD19. LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID 4035) in human. LRP-1 is single pass type I membrane protein that is expressed in most tissues, but is abundant in liver, brain, and lung. LRP1 is synthesized with a signal peptide sequence (a.a. 1-19) and is processed in trans-Golgi network by furin to generate a 515 kDa alpha subunit and an 85 kDa beta subunit. The alpha and beta subunits are non-covalently linked during LRP1 transport to the cell membrane. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (ApoE), APP and amyloid beta. It is the primary receptor mediating transport of amyloid beta peptides across the blood-brain barrier into circulation, thereby clearing them from the brain. LRP1 is required for early embryonic development and is involved in cellular lipid homeostasis and plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin). Excessive copper accumulation in the brain has been linked with reduced LRP1 mediated clearance of amyloid beta peptides across the blood brain barrier, which may contribute to complications of Alzheimers disease. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LRP1, 85 kDa subunit, clone 6F8, Cat. No. MABN1796, is a highly specific mouse monoclonal antibody that targets LRP1 85 kDa subunit and has been tested in ELISA and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected LRP1 85 kDa subunit in 10 µg of mouse hippocampus tissue lysate. ELISA Analysis: A representative lot was employed as the capture antibody for the detection of LRP1 in different human brain regions by sandwich ELISA. a strong positive correlation between LRP1 and PSD95 regional distribution was observed (Shinohara, M., et al. (2013). Acta Neuropathol. 125(4):535-547). Western Blotting Analysis: A representative lot detected siRNA-mediated LRP1 knockdown in human brain vascular pericytes (Casey, C.S., et al. 2015. J. Biol. Chem. 290(22):14208-14217). Note: The use of 5% skim milk as the blocking agent and 1-2 hr instead of overnight primary incubation time is recommended for Western blotting application to minimize non-specific background. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to the C-terminal end sequence of human/mouse LRP1. |
| Clone | 6F8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 6F8 (A.k.a. 6AF8) epitope lies within LRP1 C-terminal end sequence present in the membrane-bound beta-fragment (85 kDa subunit), but not the extracellular alpha-fragment (515 kDa subunit). Target region is 100% conserved between human and murine species, but missing in human LRP1 spliced isoform 2. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~85 kDa observed. Target band size appears larger than the calculated molecular weight of 65.78 kDa (a.a. 3944-4544; UniProt Q07954) due to glycosylation. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain and mouse hippocampus tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected LRP1 85 kDa subunit in 10 µg of mouse whole brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |