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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1773 |
| Description | Anti-oxDJ-1 Antibody (Cys106), clone M149 |
| Alternate Names |
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| Background Information | Protein DJ-1 (UniProt Q99497; also known as Parkinson disease (autosomal recessive, early onset) protein 7, Epididymis secretory sperm binding protein Li 67p, Oncogene DJ1) is encoded by the PARK7 (also known as DJ1, HEL-S-67p) gene (Gene ID 11315) in human. DJ-1 belongs to the ThiJ/PfpI family of proteins characterized by a ThiJ domain. DJ-1 is reported to be involved in multiple physiological processes, including cellular oncogenic transformation, RNA-protein interaction regulation, sperm fertilization, antioxidative defense, and transcriptional regulation. Mutations in the DJ-1 gene, PARK7, are known causes of autosomal recessive parkinsonism with a clinical presentation similar to other forms of recessive PD syndromes. DJ-1 cysteine residue 106 (Cys106) is preferentially oxidized through direct oxygen addition in response to cellular oxidative stress. Cysteine forms 3 different oxidized species, cysteine–sulfenic acid (Cys-SOH), cysteine–sulfinic acid (Cys-SO2H), and cysteine–sulfonic acid (Cys-SO3H). The Cys-SO2H form of oxDJ-1 is likely the active form, and further oxidation to Cys-SO3H leads to loss of biologic function. The levels of oxDJ-1 in erythrocytes from unmedicated PD patients are reported to be markedly higher than those from medicated PD patients and healthy subjects. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-oxDJ-1 Antibody (Cys106), clone M149 is validated for use in Western Blotting and Immunohistochemistry for the detection of oxDJ-1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A reporesentative lot detected DJ-1 oxidation in various regions of murine (cerebral cortex, hippocampus, striatum, SN pars compacta, SN pars reticulata) and human (SN and red nucleus of the midbrain) brain tissue sections (Saito, Y., et al. (2014). J Neuropathol Exp Neurol. 73(7):714-728.) Western Blotting Analysis: A reporesentative lot detected basal DJ-1 oxidation (oxDJ-1) in human neuroblastoma SH-SY5Y cells and murine fibroblasts from wild-type, but not DJ-1 knockout, mice, as well as enhanced oxDJ-1 in H2O2-treated SH-SY5Y cells (Saito, Y., et al. (2014). J Neuropathol Exp Neurol. 73(7):714-728.). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged oxidized recombinant DJ-1 purified from H2O2-treated E. coli host corresponding to the Cys106-SO2H/-SO3H of human oxDJ-1. |
| Epitope | Cys106-SO2H/-SO3H |
| Clone | M149 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Immunoreactivity toward Cys-SO2H or Cys-SO3H form of oxDJ-1 is not yet determined. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~20 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: 0.1 µg/mL of this antibody detected enhanced DJ-1 oxidation in 10 µg cell lysate from H2O2-treated human neuroblastoma SH-SY5Y cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |