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Millipore This Huc/Hud Antibody Is Validated For Use In Wb & Ihc For The Detection Of The Huc/Hud Protein. - Mill (Additional S&H or Hazmat Fees May Apply)
Hu proteins are mammalian embryonic lethal abnormal visual system (ELAV)-like neuronal RNA-binding proteins that contain three RNA recognition motifs. RNA binding protein HuC promotes neuronal development in both the central and the peripheral nervous systems. HuC antibodies are used as markers for developing neurons. HuD may play a role in neuron-specific RNA processing, and be involved in maintaining the proliferative state of cells.
Product Information
Format
Purified
Control
Human brain tissue lysate
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This HuC/HuD antibody is validated for use in WB & IHC for the detection of the HuC/HuD protein.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected HuC/HuD in human brain and rat hippocampus tissues.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human HuC/HuD.
Clone
15A7.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Rat
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001411
Gene Symbol
ELAVL3
HUC
PLE21
ELAVL4
HUD
PNEM
Purification Method
Protein G Purified
UniProt Number
Q14576
P26378
Molecular Weight
~ 40 kDa observed. An uncharacterized band may appear at ~45 kDa in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HuC/HuD in 200 µg of human brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
