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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1529 |
| Description | Anti-PRICKLE2 Antibody, clone 3B4.1 |
| Alternate Names |
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| Background Information | Prickle-like protein 2 (UniProt Q7Z3G6) is encoded by the PRICKLE2 (also known as EPM5) gene (Gene ID 166336) in human. Prickle2 is a nuclear membrane associated protein that belongs to a highly conserved Prickle, Espinas, Testin (PET) and Linl-1, Isl-1, and Mec-3 (LIM) domain-containing protein family. Prickle is part of the non-canonical Wnt signaling pathway that establishes planar cell polarity. It plays a critical role in neuron architecture and function. In mice it is shown to be highly expressed in the hippocampus region where it localizes to the post-synaptic density (PSD). It interacts the PSD-95 and NMDA receptor, the two proteins implicated in Autism spectrum disorders. Loss of Prickle2 is known to reduce number and size of miniature synaptic currents, indicating deficits in basal synaptic transmission. Mice deficient in Prickle2 exhibit lower seizure threshold and PRICKLE2 mutations in humans are associated with progressive myoclonic epilepsy type 5. Ref.: Sowers, LP., et al.. (2013), Mol. Psychiatry. 18, 1077-1089. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PRICKLE2 Antibody, clone 3B4.1, Cat. No. MABN1529, is a highly specific mouse monoclonal antibody that targets PRICKLE2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PRICKLE2 in mouse cerebral cortex, as well as in human pancreas, small intestine, and tonsil tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human PRICKLE2 internal fragment. |
| Clone | 3B4.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3B4.1 targets an internal PRICKLE2 epitope conserved between human and murine species. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~95 kDa observed. 95.62/95.30 kDa (human pro-/mature form) and 95.78/95.47 kDa (mouse pro-/mature form) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected PRICKLE2 in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |