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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | ICC, WB, IHC | Rb | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN140 |
| Description | Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4 |
| Background Information | NeuN is a RNA-binding protein that belongs to the evolutionarily conserved Fox-1 family. It is mainly involved RNA splicing. It is found exclusively in the nuclei of neuronal cells, but is also present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although. Some neurons fail to recognize NeuN at all ages in certain cells. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4 detects level of NeuN (rabbit monoclonal) & has been published & validated for use in ICC, Western Blotting & IHC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 cortex and hippocampus primary cultures. Immunohistochemistry Analysis: A 1:400 dilution of this antibody detected NeuN in mouse cerebellum and hippocampus tissues. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to mouse NeuN. |
| Clone | 27-4 |
| Host | Rabbit |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with mouse and rat. Predicted to react with human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein A Purfied |
| Molecular Weight | ~46/48 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse brain nuclear extract. Western Blot Analysis: A 1:2,000 dilution of this antibody detected NeuN in 10 µg of mouse brain nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |