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Millipore Detect Mglur5 Using This Mouse Monoclonal Antibody, Anti-Mglur5 Antibody, Clone 11d9.1 Validated For Use In Western Blotting & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
Metabotropic glutamate receptor 5 (mGluR5) is a member of the G-protein coupled receptor family and functions as a receptor for glutamate. mGluR5 activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.
Product Information
Format
Purified
Control
Rat brain micropreparation tissue lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect mGluR5 using this mouse monoclonal antibody, Anti-mGluR5 Antibody, clone 11D9.1 validated for use in western blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected mGluR5 in normal human brain tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human mGluR5.
Clone
11D9.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Rat
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000833
Gene Symbol
GRM5
GPRC1E
MGLUR5
Purification Method
Protein G Purified
UniProt Number
P41594
Molecular Weight
~150 kDa observed. The calculated molecular weight is 130 kDa, however mGluR5 has been shown as a ~150 kDa band in western blots (Romano, C., et al. (1996). J Biol Chem. 271(45):28612-28616).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in rat brain micropreparation tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected mGluR5 in 10 µg of rat brain micropreparation tissue lysate. The lysate was not boiled during preperation for western blotting.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
