Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1193 |
| Replaces | MAB368 |
| Description | Anti-Synaptophysin Antibody, clone 10F6.1 |
| Alternate Names |
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| Background Information | Synaptophysin (UniProt: P07825; also known as Major synaptic vesicle protein p38) is encoded by the Syp gene (Gene ID: 24804) in rat. Synaptophysin belongs to the synaptophysin/synaptobrevin family of proteins. It is one of the major glycoproteins present in presynaptic vesicles and in various neuroendocrine cells of both neuronal and epithelial phenotype. Its structure consists of a hexameric protein of four transmembrane spans with a short cytoplasmic amino and a long carboxy-terminal tail. Both amino- and carboxy-terminals are exposed on the cytoplasmic surface of the synaptic vesicle membrane. C-terminal domain of Snaptophysin is selectively required for the endocytosis during, but not after, cessation of sustained synaptic transmission. Synaptophysin is involved in organizing membrane components may regulate endocytosis to ensure vesicle availability following sustained neuronal activity. It is also involved in the regulation of short-term and long-term synaptic plasticity. Syp / neurons display functional deficiencies and slower recovery of the recycling synaptic vesicle pool. (Ref.: Kwon, SE and Chapman, ER (2011). Neuron. 70(5): 847 854). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Synaptophysin Antibody, clone 10F6.1, Cat. No. MABN1193, is a highly specific monoclonal antibody that targets Synaptophysin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Synaptophysin in human cerebellum, human cerebral cortex, and human pancreatic tissues. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fragment corrsponding to 86 amino acids from the C-terminal region of rat synaptophysin. |
| Clone | 10F6.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 10F6.1 detects synaptophysin in rat, human, and mouse brain. It targets a sequence within amino acids 222-307 in rat synaptophysin. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~40 kDa observed; 33.31 kDa calculated. Glycosylated form displays higher molecular weight (~38 kDa). Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: A 1:50,000 dilution of this antibody detected Synaptophysin in 10 µg of rat brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |