Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | AI, FC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF950 |
| Description | Anti-Integrin alpha-4 (CD49d) Antibody, clone TA-2 |
| Alternate Names |
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| Background Information | Integrin alpha-4 (UniProt: D3ZMQ3; also known as CD49d, Protein Itga4) is encoded by the Itga4 gene in rat. Integrin-Alpha 4 contains 7 GF-GAP repeats. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with either beta-1 or beta-7 to generate a heterodimer. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Integrin alpha-4/beta-1 (alpha 4/CD29; VLA-4) may regulate leukocyte to endothelial cells adhesion by controlling Junctional adhesion molecules-L homodimerization. Integrins alpha-4/beta-1 and alpha-4/beta-7 are receptors for fibronectin and they recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-4/beta-1 plays a major role in the regulation of immune cell recruitment to inflamed endothelia and sites of inflammation. On activated endothelial cells integrin alpha-4/beta-1 triggers homotypic aggregation for most alpha-4/beta-1-positive leukocyte cell lines. This monoclonal antibody (clone TA-2) is shown to inhibit rat lymphocyte adhesion to stimulated HUVEC. It also blocks adhesion small peritoneal exudate lymphocytes (sPEL) adhesion to TNF-alpha and IL-1 stimulated human umbilical vein endothelial cells. (Ref.: Issekutz, TB., and Wykretowicz, A (1991). J. Immunol. 147:109-116). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Integrin alpha-4 (CD49d), clone TA-2, Cat. No. MABF950 is tested for use in Flow Cytometry, Immunoprecipitation, and in functional studies as Agonist or Inhibitor for the detection of CD49d/Integrin alpha-4. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Flow Cytometry appications (Latta, M., et. al. (2007). Immunology. 121(4):555-64). Flow Cytometry Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Flow Cytometry appications (Schlager, C., et. al. (2016). Nature. 530(7590):349-53). Agonist or Inhibitor Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Agonist or Inhibitor appications (Issekutz, T.B., et. al. (1991). J Immunol. 147(12):4178-84). Agonist or Inhibitor Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Agonist or Inhibitor appications (Odoardi, F., et. al. (2012). Nature. 488(7413):675-9). Agonist or Inhibitor Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Agonist or Inhibitor appications (Schlager, C., et. al. (2016). Nature. 530(7590):349-53). Immunoprecipitation Analysis: A representative lot detected Integrin alpha-4 (CD49d) in Immunoprecipitation appications (Schlager, C., et. al. (2016). Nature. 530(7590):349-53). |
| Biological Information | |
|---|---|
| Immunogen | Small peritoneal exudate lymphocytes (sPELs). |
| Clone | TA-2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone TA-2 detects CD49d/Integrin alpha-4 in Rat splenocytes and lymphocytes. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Rat. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 115.59 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in rat splenocytes and lymphocytes. Flow Cytometry Analysis: 1 µg of this antibody detected Integrin alpha-4 (CD49d) in one million rat splenocytes and lymphocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 ug |