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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | PE-Cy7 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF402 |
| Description | Anti-CD27 Antibody (human), PE-Cy7, clone O323 |
| Alternate Names |
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| Background Information | The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells.The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naïve vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD27, CD1d, IL-10 and TGF-beta (Kessel et al. 2012. Autoimm. Rev. 11(9): 670-677). The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27. |
| Product Information | |
|---|---|
| Format | PE-Cy7 |
| Presentation | Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal antibody Anti-CD27 (human), PE-Cy7 & clone O323 validated for use in Flow Cytometry for the detection of CD27. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Immunogen information for this antibody is proprietary information. |
| Clone | O323 |
| Concentration | 5 µL (0.25 µg)/test |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Size Exclusion Chromatography |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Flow Cytometry Analysis: 0.25 µg of this antibody detected CD27 in one million human peripheral blood lymphocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Packaging Information | |
|---|---|
| Material Size | 100 tests |