Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ca, H | FC, IHC, IP, AI, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2033 |
| Description | Anti-CD31/PECAM-1.3 Antibody, clone MBC 78.3 |
| Alternate Names |
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| Background Information | Platelet endothelial cell adhesion molecule (UniProt: P16284; also known as PECAM-1, EndoCAM. GPIIA, PECA1, CD31) is encoded by the PECAM1 gene (Gene ID: 5175) in human. PECAM-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved to generate the mature protein with a large extracellular region (aa 28-601) that contains six Ig-like C2-type domains, followed by a transmembrane segment (aa 602-620) and a cytoplasmic tail (aa. 621-738 of isoform Long) that plays an essential role in PECAM-1-mediated cellular signaling. PECAM-1 is essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Six isoforms of PECAM-1 have been described that are produced by alternative splicing. Isoform Long predominates in all tissues examined, whereas Delta 12 isoform is detected only in trachea and isoform Delta14-15 are only detected in lung tissue. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia and U-937 histiocytic lymphoma cell lines. Tyrosine 690 residue plays a critical role in TEM and is required for efficient trafficking of PECAM-1 to and from the lateral border recycling compartment (LBRC) and is also required for the LBRC membrane to be targeted around migrating leukocytes. PECAM-1 also prevents phagocyte ingestion of closely apposed viable cells by transmitting detachment signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD31/PECAM-1.3, clone MBC 78.3 , Cat. No. MABF2033 is a mouse monoclonal antibody that detects PECAM1 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist or Inhibitor function, and Western Blotting. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected CD31/PECAM-1.3 in Flow Cytometry applications (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8). Flow Cytometry Analysis: 1 µg from a representative lot detected CD31/PECAM-1.3 in THP-1 cells. Western Blotting Analysis: A representative lot detected CD31/PECAM-1.3 in Western Blotting applications (Zhao, T., et. al. (2001). J Cell Biol. 152(1):65-73). Agonist or Inhibitor Analysis: A representative performed agonist or Inhibitor function (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8; Reedguist, K.A., et. al. (2000). J Cell Biol. 148(6):1151-8). Immunoprecipitation Analysis: A representative lot detected CD31/PECAM-1.3 in Immunoprecipitation applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27). Immunocytochemistry Analysis: A representative lot detected CD31/PECAM-1.3 in Immunocytochemistry applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27; Zhao, T., et. al. (2001). J Cell Biol. 152(1):65-73). |
| Biological Information | |
|---|---|
| Immunogen | PECAM-1 purified from human platelets. |
| Epitope | extracellular domain |
| Clone | MBC 78.3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone MBC 78.3 detects CD31/PECAM-1 in human and canine cells. It targets an epitope within the IgG homology domain 1. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Canine, Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 82.54 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in un-permeablilized THP-1 cells. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CD31/PECAM-1.3 in un-permeablilized THP-1 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |