Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Gp, H | FC, IHC, Inhibition, Neutralization Assay | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2031 |
| Description | Anti-CD1b Antibody, clone BCD1b3.1 |
| Alternate Names |
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| Background Information | T-cell surface glycoprotein CD1b (UniProt: P29016; also known as CD1b) is encoded by the CD1B gene (Gene ID: 910) in human. CD1b is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. The most prominent known function of CD1b molecules is to associate with lipids from pathogens in order to stimulate T cells and induce host defense mechanisms. CD1b is expressed on cortical thymocytes, certain T-cell leukemia, and other tissues. Two isoforms of CD1b are reported that are produced by alternative splicing. CD1b has an extracellular domain (aa 18-3-3), a helical domain (aa 304-324), and a cytoplasmic tail (aa 329-332). The newly synthesized CD1b molecules follow an intracellular transport pathway that leads it to the cell surface from endoplasmic reticulum and subsequently to internalization from the plasma membrane into the endocytic pathway. After internalization, CD1b molecules are sorted for transport to lysosomes in the sorting endosome. (Ref.: Briken, V., et al. (2002). EMBO J. 21(4); 825-834). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD1b, clone BCD1b3.1, Cat. No. MABF2031, is a mouse monoclonal antibody that detects T-cell surface glycoprotein CD1b and it has been tested for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition assays, and Neutralizing function. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot detected CD1b in Immunohistochemistry applications (Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88). Flow Cytometry Analysis: A representative lot detected CD1b in Flow Cytometry applications (Hiromatsu, K., et. al. (2002). Immunology. 106(2):159-72; Torrelles, J.B., et. al. (2011). J Biol Chem. 286(41):35438-46; Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88; Sieling, P.A., et. al. (2005). J Immunol. 174(5):2637-44; Facciotti, F., et. al. (2011). Proc Natl Acad Sci USA. 108(34):14228-33). Immunoprecipitation Analysis: A representative lot detected CD1b in Immunoprecipitation applications (Briken, V., et. al. (2002). EMBO J. 21(4):825-34; Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88). Neutralizing Analysis: A representative lot detected CD1b in Neutralizing applications (Torrelles, J.B., et. al. (2011). J Biol Chem. 286(41):35438-46). Inhibition Analysis: A representative lot detected CD1b in Inhibition applications (Behar, S.M., et. al. (1995). J Exp Med. 182(6):2007-18). |
| Biological Information | |
|---|---|
| Immunogen | Isolated human CD1+ monocytes. |
| Clone | BCD1b3.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone BCD1b3.1 detects T-cell surface glycoprotein CD1b in human and guinea pig. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Guinea Pig, Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 36.94 kda calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in MOLT4 cells. Flow Cytometry Analysis: 1 µg of this antibody detected CD1b in one million MOLT4 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |