Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IHC, Immunodepletion | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF1983 |
| Description | Anti-CD8a Antibody, clone YTS 169.4 |
| Alternate Names |
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| Background Information | T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the Cd8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 is a single-pass type I membrane protein with one Ig-like V-type (immunoglobulin-like) domain. CD8a is produced with a 27 amino acid signal peptide that is subsequently cleaved. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2b in PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD8a, clone YTS 169.4, Cat. No. MABF1983 is a rat monoclonal antibody that detects T-cell surface glycoprotein CD8 alpha chain and has been tested for use in Flow Cytometry, Immunohistochemistry, and Immunodepletion. |
| Key Applications |
|
| Application Notes | Immunodepletion Analysis: A representative lot detected CD8a in Immunodepletion applications (Cobbold, S.P., et. al. (1984). Nature. 312(5994):548-51; Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202; Brown, C.R., et. al. (1990). J Immunol. 145(10):3438-41). Flow Cytometry Analysis: A representative lot detected CD8a in Flow Cytometry applications (Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202). Immunohistochemistry Analysis: A representative lot detected CD8a in Immunohistochemistry applications (Wright, J.R. Jr, et. al. (1997). Ann Transplant. 2(3):12-6). |
| Biological Information | |
|---|---|
| Immunogen | Thymocytes from CBA mouse. |
| Clone | YTS 169.4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone YTS 169.4 detects T-cell surface glycoprotein CD8 alpha chain (CD8a) in mouse thymocytes. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 27.5 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in mouse thymocytes. Flow Cytometry Analysis: 1 µg of this antibody detected CD8a in mouse thymocytes. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |