Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A Broad Range Of Species | Inhibition, ICC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF1975 |
| Description | Anti-Herpes Simplex Virus Glycoprotein D Antibody, clone LP14 |
| Alternate Names |
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| Background Information | Herpes Simplex Virus envelope Glycoprotein D (UniProt: Q69091) is also known as gD. The envelope of herpes simplex virus (HSV) is complex and contains at least 10 virus-encoded glycoproteins, some of which mediate virus entry into the host cell. HSV virus enters the cell by first fusing its envelope with a membrane of the host cell. Five viral envelope glycoproteins are reported to be involved in this entry process. Binding of glycoprotein D of HSV to a cell surface receptor is required to trigger membrane fusion during entry into host cells. Glycoproteins C and B promote attachment by interacting with cell surface proteoglycans and then glycoprotein D binds to the herpes virus entry mediator (HVEM) receptor that initiates a process that ultimately leads to glycoprotein B-mediated membrane fusion. Glycoprotein D is synthesized with a signal peptide (aa 1-25) that is subsequently cleaved off. It is a homodimeric protein that contains two zinc-binding sites (aa 64 and 264). The ectodomain region of glycoprotein D contains three sites for the addition of N-linked oligosaccharides (N-CHO) and six cysteine residues arranged into three disulfide bonds. During virion morphogenesis, glycoprotein D probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. (Ref.: Whitbeck JC et al. (1997). J. Virol. 71(8); p. 6083 6093; Nicola, AV et al. (1998). J Virol. 72(5): 3595-3601; Giovine, PD et al. (2011). PLos Pathog. 7(9); e1002277). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in PBS without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Herpes Simplex Virus Glycoprotein D, clone LP14, Cat. No. MABF1975, is a mouse monoclonal antibody that detects Glycoprotein D of Herpes Simplex Virus and has been tested for use in Inhibition, Immunocytochemistry, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Herpes Simplex Virus Glycoprotein D in lysates from VERO cells transfected with HSV-1. Western Blotting Analysis: A representative lot detected Herpes Simplex Virus Glycoprotein D in Western Blotting applications (Lau, S.Y., et. al. (2015). Viruses. 7(3):915-38; Zenner, H.L., et. al. (2011). J Virol. 85(16):8012-21; Pawliczek, T., et. al. (2009). J Virol. 83(21):11254-64). Western Blotting Analysis: A representative lot detected Herpes Simplex Virus Glycoprotein D in Western Blotting applications (Ren, Y., et. al. (2012). J Gen Virol. 93(Pt 2):319-29). Immunocytochemistry Analysis: A representative lot detected Herpes Simplex Virus Glycoprotein D in Immunocytochemistry applications (Lau, S.Y., et. al. (2015). Viruses. 7(3):915-38). Inhibition Analysis: A representative lot inhibited the fusion of cells infected with HSV-1. (Minson, A.C., et. al. (1986). J Gen Virol. 67(6); 1001-1013. |
| Biological Information | |
|---|---|
| Immunogen | Whole Herpex Simplex Virus 2 (HSV-2). |
| Clone | LP14 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone LP14 detects glycoprotein D of Herpes Simplex virus. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Virus. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~50 and 55 kDa observed; 43.35 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293T cell lysate transfected with HSV gD. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Herpes Simplex Virus Glycoprotein D in HEK293T cell lysate transfected with HSV gD. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |