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Millipore This Rat Monoclonal Anti-Mouse Ifn Gamma Antibody, Apc, Clone Xmg1.2, Cat. No. Mabf1515 Is Validated For Use In Flow Cytometry, For The Detection Of Ifn Gamma. - Mill (Additional S&H or Hazmat Fees May Apply)
The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells, which allow the cytokine to modulate innate immunity, when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein, e.g. by flow cytometry, as well as for quantitative analysis of the secreted protein by ELISA, when paired with an appropriate secondary antibody. This clone is also widely used for neutralization of the functional activity of IFN-g in a variety of assays.
Product Information
Format
APC
Presentation
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality Level
MQ100
Applications
Application
This rat monoclonal Anti-mouse IFN gamma Antibody, APC, clone XMG1.2, Cat. No. MABF1515 is validated for use in Flow Cytometry, for the detection of IFN gamma.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
XMG1.2
Concentration
Please refer to lot specific datasheet.
Host
Rat
Isotype
IgG1κ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
Ifng
Purification Method
Purified by size-exclusion chromatography
UniProt Number
P01580
Product Usage Statements
Quality Assurance
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.06 μg from a representative lot detected IFN gamma in one million C57Bl/6 splenocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
