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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | M | Protein G Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE969 |
| Description | Anti-VprBP Antibody, clone 8A7.2 |
| Alternate Names |
|
| Background Information | Protein VPRBP (EC 2.7.11.1; UniProt Q9Y4B6; also known as DDB1- and CUL4-associated factor 1, HIV-1 Vpr-binding protein, Serine/threonine-protein kinase VPRBP, Vpr-interacting protein, VprBP) is encoded by the VPRBP (also known as DCAF1, KIAA0800, RIP) gene (Gene ID 9730) in human. VprBP/DCAF1 acts as an adaptor molecule that mediates substrates recruitment for both the RING-type CRL4 (Cul4-Roc1-DDB1) and HECT-type EDD/UBR5 E3 ubiquitin ligase complexes. CRL4 substrates known to be recruited by VprBP include Merlin, Mcm10, UNG2, SMUG1, RORαme1. Known EDD/UBR5 substrates recruited by VprBP include Katanin and TERT. VprBP is involved in a variety of normal cellular processes, including proliferation, DNA replication, cell cycle progression, telomere maintenance, and DNA damage responses. As indicated by its name, VprBP is also a well known target utilized by the Vpr and Vpx accessory factors of primate lentiviruses to highjack the host CRL4VprBP E3 ligase function. |
| Product Information | |
|---|---|
| Format | Protein G Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-VprBP Antibody, clone 8A7.2 is an antibody against VprBP for use in Western Blotting, Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected VprBP in human kidney, human small intestine, and rat testis tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | BCP-conjugated linear peptide corresponding an N-terminal sequence of human VprBP. |
| Epitope | N-terminal region. |
| Clone | 8A7.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with all three spliced isoforms of human VprBP reported by UniProt (Q9Y4B6). |
| Isotype | IgG2b |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. Predicted to react with Mouse based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~169 kDa observed. 169.0 kDa (isoform 1), 168.9 kDa (isoform 2), and 118.2 kDa (isoform 3) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HCT116 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected VprBP in 10 µg of HCT116 human colon carcinoma cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |