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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, IHC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE8 |
| Description | Anti-EBNA2 Antibody, clone R3 |
| Overview | Gross, H., et al. (2010). Virology. 397(2): 299-310. PMID: 19969318 Kremmer, E., et al. (1995). Virology. 208(1): 336-342. PMID: 11831716 |
| Alternate Names |
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| Background Information | The Epstein-Barr virus or EBV is associated with several human cancers and actively immortalizes B lymphocytes. EBV is leads to expression by a small group of viral proteins, including Epstein-Barr nuclear antigen 2 (EBNA2) which is required for immortalization. EBNA2 is a transcriptional activator belonging to the herpesviridae EBNA2 family, and performs a crucial role in activating the host resting B-cell and inducing B-cell proliferation. EBNA2 possesses an acidic activating domain with which it is able to recruit various cellular and viral involved in B-cell immortalization. Implicated in cellular phenotypic modifications, EBNA2 is known to upregulate the expression of cell surface receptors, such as CD21, MYC, and CD23, as well as upregulating viral genes such as LMP1, LMP2A, LMP2B, and EBNA1-6. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-EBNA2 Antibody, clone R3 is a Rat Monoclonal Antibody for detection of EBNA2 also known as Epstein-Barr nuclear antigen 2 & has been validated in WB, IP & IHC. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Gross, H., et al. (2010). Virology. 397(2):299-310.) Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Kremmer, E., et al. (1995). Virology. 208(1):336-342.) |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human EBNA2. |
| Epitope | C-terminus of EBNA2 |
| Clone | R3 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with human cell infected with EBV. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G |
| UniProt Number |
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| UniProt Summary | FUNCTION: Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by upregulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2/M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells (By similarity). SUBUNIT STRUCTURE: Interacts with human SMARCB1/INI1, presumably generating an open chromatin conformation at the EBNA2-responsive target genes. Interacts with human WAPAL. Interacts with host CBF1; this interaction allows transcriptional activation by EBNA2. Interacts with host general transcription factors GTF2B, ERCC2 and ERCC3 (By similarity). SUBCELLULAR LOCATION: Host nucleus matrix. Note: Associated with the nuclear matrix (By similarity). PTM: Phosphorylated (By similarity). SEQUENCE SIMILARITIES: Belongs to the herpesviridae EBNA2 family. |
| Molecular Weight | ~ 75 kDa observed; Although the calculated molecular weight is 49 kDa, this protein is typically observed at ~ 75-80 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in B95-8 cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected EBNA2 on 10 µg of B95-8 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |