Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE666 |
| Replaces | MAB3584 |
| Description | Anti-Heterochromatin Protein-1 Antibody, clone 4C12.1 |
| Alternate Names |
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| Background Information | Chromobox protein homolog 5 (UniProt: P45973; also known as Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha) is encoded by the CBX5 (also known as HP1A) gene (Gene ID: 23468) in human. Chromobox protein homolog 5 is a component of centromeric and pericentromeric heterochromatin that associates with chromosomes during mitosis. Chromobox protein homolog 5 associates specifically with chromatin during metaphase and anaphase. It recognizes and binds histone H3 tails methylated at Lys9 (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when Tyr41 of histone H3 is phosphorylated. It can interact with lamin-B receptor (LBR) and this interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. It is also involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Heterochromatin Protein-1 , clone 4C12.1, Cat. No. MABE666, is a highly specific mouse monoclonal antibody that targets Heterochromatin Protein-1 alpha and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Heterochromatin Protein-1 in human tonsil, human pancreas, and human uterus tissues. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fraction corresponding to 68 amino acids from the internal region of human Heterochromatin protein 1 homolog alpha. |
| Clone | 4C12.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~23 kDa observed; 22.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NIH/3T3 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Heterochromatin Protein-1 in 10 µg of NIH/3T3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |