Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE659 |
| Description | Anti-Myb Antibody, clone 8F12.1 |
| Alternate Names |
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| Background Information | Transcriptional activator Myb (UniProt: P10242; also known as Myb, Proto-oncogene c-Myb) is encoded by the MYB gene (Gene ID: 4602) in human. Myb is a transcriptional activator; DNA-binding protein that specifically recognize the sequence 5-YAAC[GT]G-3 and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. It is negatively regulated by microRNA-155. It consists of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain, and a C-terminal domain involved in transcriptional repression. MYB gene is aberrantly expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Myb protein is also reported to bind to HTLV-I long terminal repeat (LTR) sequences at four distinct regions in a sequence specific manner and activates LTR-mediated transcription. (Ref.: Dasgupta, P., et al. (1992). J. Virol. 66(1): 270-276). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Myb, clone 8F12.1 Antibody, Cat. No. MABE659, is a highly specific mouse monoclonal antibody that targets Myb and has been tested in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST/His-taged recombinant protein fragment corresponding to 154 amino acids from the C-terminal region of human Transcriptional activator Myb. |
| Clone | 8F12.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 8F12.1 specifically detects human Myb protein. It targets an epitope within 154 amino acids in the C-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~80 kDa observed; 72.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Myb in 10 µg of Jurkat cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |