| Description | |
|---|---|
| Catalogue Number | MABE463-25UG |
| Description | Anti-methylated TLS/FUS Antibody, clone 9G6 |
| Alternate Names |
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| Background Information | RNA-binding protein FUS (UniProt: also known as 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein) is encoded by the FUS (also known as TLS) gene (Gene ID: 2521) in human. Fused in sarcoma/translocated in liposarcoma FUS/TLS is a multi-functional nuclear protein that binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in super helical double-stranded DNA. It is involved in a variety of cellular functions including transcription, protein translation, RNA splicing, and transport and may play a role in maintenance of genomic integrity. A chromosomal aberration involving FUS is shown to cause of acute myeloid leukemia (AML). Mutations in FUS gene have been linked to Amyotrophic lateral sclerosis 6, with or without frontotemporal dementia that affects upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. (Ref.: Lagier-Tourenne, C., et al. (2010). Hum. Mol. Genet. 19(1); R46-R64). |
| Product Information | |
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| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-methylated TLS/FUS, clone 9G6, Cat. No. MABE463-25UG, is a highly specific rat monoclonal antibody, that targets RNA-binding protein FUS and has been tested in Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: 0.05 ug/mL frrom a representative lot detected methylated TLS/FUS in 10 µg of HeLa cell lysate. Western Blotting Analysis: 0.05 ug/mL frrom a representative lot detected methylated TLS/FUS in 10 µg of lysate from HeLa cells pretreated with AdOx. Western Blotting Analysis: A representative lot detected methylated TLS/FUS in Western Blotting applications (Suárez-Calvet, M., et. al. (2016). Acta Neuropathol. 131(4):587-604 ). |
| Biological Information | |
|---|---|
| Immunogen | Ovalbumin-conjugated linear peptide corresponding to 31 amino acids from the C-terminal region corresponding to the RGG3 domain of FUS (with all arginines methylated). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 9G6 detects methylated RNA-binding protein FUS in human cells. It targets an epitope with in 31 amino acids from the C-terminal region. |
| Isotype | IgG2b |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 70 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 0.05 ug/mL of this antibody detected methylated TLS/FUS in 10 µg of HepG2 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |