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Millipore Detect Holliday Junction Recognition Protein Using This Mouse Monoclonal Antibody, Anti-Hjurp Antibody, Clone 144k/B1 Validated For Use In Western Blotting & Ip. - Mill (Additional S&H or Hazmat Fees May Apply)
Holliday junction recognition protein (HJURP) is also known as 14-3-3-associated AKT substrate (FAKTS), Fetal liver-expressing gene 1 protein (FLEG1) and Up-regulated in lung cancer 9 (URLC9). HJURP is a centromeric protein that acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. HJURP is highly expressed in the thymus, testis, and in many lung cancer samples with lower levels found in placenta, small intestine, liver, skeletal muscle, and colon.
Product Information
Format
Purified
Control
HEK293 cell lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Holliday junction recognition protein using this mouse monoclonal antibody, Anti-HJURP Antibody, clone 144K/B1 validated for use in western blotting & IP.
Key Applications
Western Blotting
Immunoprecipitation
Chromatin Immunoprecipitation (ChIP)
Application Notes
Immunoprecipitation Analysis: A representative lot immunoprecipitated HJURP in HeLa nuclear extract (CNIO, Madrid, Spain).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot immmunoprecipitated HJURP in HEK293 cell lysate (CNIO, Madrid, Spain).
Biological Information
Immunogen
Recombinant protein corresponding to human HJURP.
Clone
144K/B1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_060880.3
Gene Symbol
HJURP
FAKTS
FLEG1
URLC9
Purification Method
Protein G Purified
UniProt Number
Q8NCD3
Molecular Weight
~90 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HJURP in 10 µg of HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
