The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Millipore This Anti-Puromycin, Clone 17h1 Is Validated For Use In Immunocytochemistry For The Detection Of Puromycin. - Mill (Additional S&H or Hazmat Fees May Apply)
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3 end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. This product is a conjugated version of Cat. No. MABE341.
Product Information
Format
AlexaFluor®488
Presentation
Purified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Quality Level
MQ100
Applications
Application
This Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.
Key Applications
Immunocytochemistry
Biological Information
Immunogen
Puromycin-incorporated neosynthesized proteins at multiple molecular weights
Clone
17H1
Concentration
0.5 mg/mL
Host
Rat
Specificity
This antibody detects Puromycin-incorporated neosynthesized proteins.
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
Antibody Type
Monoclonal Antibody
Purification Method
Protein G Purified
Molecular Weight
Refer to Cat. No. MABE341 for observed molecular weight information.
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.
Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
