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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, FC, IP, IF | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE320 |
| Replaces | 04-1127 |
| Description | Anti-c-ErbB2/c-Neu (Ab-5) Antibody, clone TA-1 |
| Alternate Names |
|
| Background Information | The ErbB2 (HER2, c-Neu, ErbB2, c-erbB2, or HER2/neu) protein is member of the human epidermal growth factor receptor (HER) family. It is a receptor tyrosine kinase containing an intracellular kinase domain and an extracellular ligand domain, although no endogenous ligand has yet been found. ErbB2 dimerizes with ErbB1, 3, and 4, and can activate different pathways including the PI3K, PLCγ, and MAPK pathways, depending on the ErbB receptor involved. Coupling with ErbB3 activates the PI3K pathway, whereas coupling with any of these three receptors can induce the MAPK pathway. ErbB2 signaling promotes cell proliferation and inhibits apoptosis. ErbB2 is overexpressed in various cancers and may play a role in the development and metastasis of gliomas, and ovarian, breast, lung, and gastric cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ cultured supernatent in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-c-ErbB2/c-Neu (Ab-5) Antibody, clone TA-1 is validated for use in Western Blotting, Flow Cytometry, Immunoprecipitation, Immunofluorescence for the detection of c-ErbB2/c-Neu (Ab-5). |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot from an independent laboratory detected c-ErbB2/c-Neu in HER-2 expressing SK-BR-3 cells (Montgomery, R. B., et al. (2005). Cancer Res. 65(2):650-656.). Western Blot Analysis: A representative lot from an independent laboratory detected c-ErbB2/c-Neu in HER-2 expressing SK-BR-3 cells (Montgomery, R. B., et al. (2005). Cancer Res. 65(2):650-656.). Immunoprecipitation Analysis: A representative lot from an independent laboratory precipitated c-ErbB2/c-Neu in IP (McKenzie, S. J., et al. (1989). 4(5):543-548.). Immunofluorescence Analysis: A representative lot from an independent laboratory detected c-ErbB2/c-Neu in IF (McKenzie, S. J., et al. (1989). 4(5):543-548.). |
| Biological Information | |
|---|---|
| Immunogen | Cells overexpressing human c-ErbB2/c-Neu |
| Epitope | Extracellular domain |
| Clone | TA-1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes the extracellular domain of ErbB2/c-Neu. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | 138 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in SK-BR-3 cells. Flow Cytometry Analysis: 0.5 µg of this antibody detected c-ErbB2/c-Neu in 0.5X10E6 SK-BR-3 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |