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Millipore Detect 5-Hydroxymethylcytosine (5hmc) With Anti-5-Hydroxymethylcytosine (5hmc) Antibody, Clone Hmc 31 (Mouse Monoclonal Antibody), That Has Been Shown To Work In Db, Medip, Elisa. - Mill (Additional S&H or Hazmat Fees May Apply)
5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.
Product Information
Format
Purified
Control
Unmodified and modified cytosine DNA.
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect 5-hydroxymethylcytosine (5hmC) with Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 (Mouse Monoclonal Antibody), that has been shown to work in DB, MeDIP, ELISA.
Key Applications
Dot Blot
Methylated DNA Immunoprecipitation (MeDIP)
ELISA
Application Notes
ELISA Analysis: A representative lot detected 5-hydroxymethylcytosine in ELISA.
Methylated DNA Immunoprecipitation (MeDIP) Analysis: A representative lot immunoprecipitated 5-hydroxymethylcytosine in ChIP.
Biological Information
Immunogen
BSA-conjugated 5-hydroxymethylcytosine.
Epitope
5-hydroxymethylcytosine
Clone
HMC 31
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes the 5-hydroxymethylcytosine of 5-Hydroxymethylcytosine DNA.
Isotype
IgG1κ
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Purification Method
Protein G Purified
Molecular Weight
This antibody detects 5-hydroxymethylcytosine.
Product Usage Statements
Quality Assurance
Evaluated by Dot Blot in unmodified and modified cytosine DNA.
Dot Blot Analysis: 0.1 µg/mL of this antibody detected 5-hydroxymethylcytosine in 10 ng and 50 ng of unmodified and modified cytosine DNA.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
