Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE243-I |
| Replaces | MABE243 |
| Description | Anti-Histone H3. X/Y Antibody, clone 8H6-2111 |
| Alternate Names |
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| Background Information | Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. It is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. Both H3.X and H3.Y are two novel primate-specific Histone H3 variants that are not found other mammals or lower eukaryotes. H3.X and H3.Y mRNAs are expressed in transformed cell lines, such as U2OS, and in low amounts in human bone, breast and lung tissue. They exhibit amino acid sequence homology with other H3 variants, but differ in several functionally important residues, such as serine10 (S to A) and Serine 28 (S to R). Knockdown of H3.Y variant is shown to affect cell growth and leads to changes in the expression of many genes involved in cell cycle control. It is noteworthy that the number of cells expressing H3.Y is increased under stress stimuli.(Ref.: Wiedemann, SM et al. (2010). J. Cell Biol. 90(5); 777-791). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Histone H3. X/Y Antibody, clone 8H6-2111, Cat. No. MABE243-I, is a highly specific rat monoclonal antibody that targets Histone H3. X/Y and has been tested in Immunocytochemistry. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Ovalbumin-conjugated linear peptide corresponding to a sequence of 12 amino acids from the N-terminal region of human Histone H3.X/Y. |
| Clone | 8H6-2111 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 8H6-2111 specifically detects X and Y variants of Histone H3 in primates. It does not recognize HA-H3.1, -H3.2, or -H3.3. This clone targets an epitope of 12 amino acids in the N-terminal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with most primates based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 17 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in U2OS cells with and without serum starvation. Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Histone H3. X/Y in U2OS cells with and without serum starvation. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |