Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ChIP | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1803 |
| Description | Anti-SPT5 Antibody, clone 6F1 |
| Alternate Names |
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| Background Information | Transcription elongation factor SPT5 (UniProt: O00267; also known as hSPT5, DRB sensitivity-inducing factor 160 kDa subunit, DSIF p160, DRB sensitivity-inducing factor large subunit, DSIF large subunit, Tat-co-transactivator 1 protein, Tat-CT1 protein) is encoded by the SUPT5H (also known as SPT5, SPT5H) gene (Gene ID: 6829) in human. SPT5 is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF is a heterodimer of SPT4 and SPT5 that positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing can facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II, which binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3 terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. SPT5 can be methylated by PRMT1/HRMT1L2 and PRMT5/SKB1 and this methylation negatively regulates interaction with the positive transcription elongation factor (P-TEFb) and RNA polymerase II. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SPT5, clone 6F1, Cat. No. MABE1803, is a highly specific rat monoclonal antibody that targets Transcription elongation factor SPT5 and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SPT5 in 10 µg of HEK293T cell lysate. Chromatin Immunoprecipitation Analysis: A representative lot detected SPT5 in Chromatin Immunoprecipitation applications (Kelso, T.W., et. al. (2014). Mol Cell Biol. 34(19):3675-88). |
| Biological Information | |
|---|---|
| Immunogen | Ovallbumin-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Transcription elongation factor SPT5. |
| Clone | 6F1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 6F1 specifically detects SPT5 in HEK293T and Jurkat cells. It targets an epitope of 18 amino acids within the C-terminal half. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~121 kDa observed; 121 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected SPT5 in 10 µg of Jurkat cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |