Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), ICC, IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1801 |
| Description | Anti-LMO2 (Rhombotin-2) Antibody, clone 299B |
| Alternate Names |
|
| Background Information | Rhombotin-2 (UniProt: P25791; also known as Cysteine-rich protein TTG-2, LIM domain only protein 2, LMO-2, T-cell translocation protein 2) is encoded by the LMO2 (also known as RBTN2, RBTNL1, RHOM2, TTG2) gene (Gene ID: 4005) in human. Rhombotin-2 is 158 amino acids, small cysteine-rich protein with multiple functions. A large portion of its structure is occupied by tandemly repeated cysteine-rich zinc-finger binding motifs, the LIM domains. However, it does not have any identifiable DNA-binding motifs. It is found predominantly in the nucleus or in the cytoplasm depending upon the tissue and the cell type. In kidney it is predominantly localized in the cytoplasm, but in hematopoietic cells it has nuclear localization. LMO2 is reported to be a proto-oncogene only in the context of T lymphocytes and is reported to play a role in T-cell leukemogenesis. It also has distinct functions in erythropoiesis. It acts with TAL1/SCL to regulate red blood cell development and interacts with LDB1 to maintain erythroid precursors in an immature state. It is reported to interact with retinoblastoma-binding protein 2 (RBP2) and human brain expressed X-linked (Bex2), both of which are shown to enhance its transcriptional activity. A chromosomal aberration involving LMO2 has been linked to T-cell acute lymphoblastic leukemia (T-ALL). (Ref.: Mao S., et al. (1997) Oncogene 14, 1531-1539). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibodyIgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LMO2 (Rhombotin-2), clone 299B Antibody, Cat. No. MABE1801, is a highly specific mouse monoclonal antibody that targets Rhombotin-2 and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected LMO2 (Rhombotin-2) in human tonsil, human placenta, and human lymphoma tissue. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected LMO2 (Rhombotin-2) in human tonsil tissues (Courtesy of Giovanna Roncador, Ph.D., Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain). Immunohistochemistry Analysis: A 1:10 dilution from a representative lot detected LMO2 (Rhombotin-2) in human tonsil and bone marrow tissues (Courtesy of Giovanna Roncador, Ph.D., Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain). Western Blotting Analysis: A representative lot detected LMO2 (Rhombotin-2) in OCILY19, SUDHL6, HEK/LMO2, and Ramos cell lysates (Courtesy of Giovanna Roncador, Ph.D., Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain). Immunocytochemistry Analysis: A representative lot detected LMO2 (Rhombotin-2) in HEK-V5-LMO2 transfected cells (Courtesy of Dr. Yasodha Natkunam, Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA). |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged full length human recombinant LMO2/Rhombotin-2. |
| Clone | 299B |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 299B detects LMO-2/Rhombotin-2 in human tissues. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G purified |
| UniProt Number |
|
| Molecular Weight | ~18 kDa observed; 18.36 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human bone marrow tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected LMO2 (Rhombotin-2) in human bone marrow tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |