Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | ICC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1796 |
| Description | Anti-YL-1 Antibody, clone EX-1Y32 |
| Alternate Names |
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| Background Information | Vacuolar protein sorting-associated protein 72 homolog (UniProt: Q15906; also known as Protein YL-1, Transcription factor-like 1) is encoded by the VPS72 (also known as TCFL1, YL1) gene (Gene ID: 6944) in human. YL-1 is a nuclear protein that may serve as a DNA-binding transcriptional regulator and may be involved in chromatin modification and remodeling. YL-1 is plays multiple roles in chromatin modification and remodeling in cells and it is shown to be a subunit of TRRAP/TIP60 HAT complex and of chromatin remodeling SCCAP-containing complex. It has a DNA binding region that includes amino acids 156 to 206. In addition, it has an aspartic/glutamic acid rich acidic region (aa 22-77) and a proline rich region (aa 333-354). Two isoforms of YL-1 have been described that are produced by alternative splicing. (Ref.: Cai, Y., et al. (2006). J. Biol. Chem. 280 (14); 13665-70). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide and 40% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-YL-1, clone EX-1Y32, Cat. No. MABE1796, is a highly specific mouse monoclonal antibody that targets Vacuolar protein sorting-associated protein 72 homolog and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected YL-1 in Western Blotting applications (Obri, A., et. al. (2014). Nature. 505(7485):648-53). Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected YL-1 in HeLa cells. Western Blotting Analysis: 2 µg/mL from a representative lot detected YL-1 in 10 µg of HeLa cell lysate. Immunoprecipitation Analysis: 1-10 µg from a representative lot detected YL-1 in Mouse embryonic fibroblast (MEF) total cell extracts. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged full-length recombinant human YL-1 protein. |
| Clone | EX-1Y32 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone EX-1Y32 detects Vacuolar protein sorting-associated protein 72 homolog (YL-1) in human and murine cells. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A + Protein G |
| UniProt Number |
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| Molecular Weight | ~48 kDa observed, 40.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Mouse embryonic fibroblast (MEF) cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected YL-1 in Mouse embryonic fibroblast (MEF) cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |