Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Yeast | WB, ChIP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1785 |
| Description | Anti-Ubiquitinated Histone H2B (Lys123) Antibody, clone 1B3F12/A9 |
| Alternate Names |
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| Background Information | Histone H2B.2 (UniProt: P02294) is encoded by the HTB2 (also known as H2B2) gene (Gene ID: 852284) in Saccharomyces cerevisiae (yeast). Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the beads on a string structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Histone H2B is phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase and may be correlated with chromosome condensation. Histone H2B can undergo acetylation by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Ubiquitinated Histone H2B (Lys123), clone 1B3F12/A9 Antibody, Cat. No. MABE1785, is a highly specific mouse monoclonal antibody, that targets ubiquitinated Histone H2B and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2 µg/mL from a representative lot detected Ubiquitinated Histone H2B (Lys123) in wild type whole cell yeast extracts and ubp8 knockout whole cell yeast extracts. Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Ubiquitinated Histone H2B (Lys123) in H2BK123ub relative to H2B at the coding sequence of the SPA2 gene in budding yeast (relative to WT) (Courtesy of Fred van Leeuwen, Ph.D., Division of Gene Regulation B4, The Netherlands Cancer Institute Plesmanlaan 121, Amsterdam Netherlands). |
| Biological Information | |
|---|---|
| Immunogen | Conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of yeast Histone H2B ubiquitinated at Lysine 123. |
| Clone | 1B3F12/A9 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 1B3F12/A9 detects K123 ubiquitinated histone H2B in human and yeast. It targets an epitope within 16 amino acids from the C-terminal region of ubiquitinated yeast Histone H2B. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Yeast |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~23 kDa observed; 14.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell acid extract. Western Blotting Analysis: 2 µg/mL of this antibody detected Ubiquitinated Histone H2B (Lys123) in HeLa cell acid extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |