Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1671 |
| Description | Anti-CCAR1 Antibody, clone 4B9.1 |
| Alternate Names |
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| Background Information | Cell division cycle and apoptosis regulator protein 1 (UniProt: Q8IX12; also known as Cell cycle and apoptosis regulatory protein 1, CARP-1, Death inducer with SAP domain, CCAR1) is encoded by the CCAR1 (also known as CARP1, DIS) gene (Gene ID: 55749) in human. CCAR1 is a cytoplasmic protein that localized to the perinuclear region. It is expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Its expression is shown to be regulated by growth factors. Two isoforms of CCAR1 have been reported that are generated by alternative splicing. CCAR1 associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. CCAR1 is recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. It also functions as a p53 coactivator and may play a role in transcriptional regulation. It is reported to be involved in apoptosis signaling in the presence of the reinoid CD437. In association with CALCOCO1, it enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells. CCAR1 can act as a coactivator and corepressor of AR-mediated transcription and contribute to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CCAR1, clone 4B9.1, Cat. No. MABE1671, is a highly specific mouse monoclonal antibody that targets Cell division cycle and apoptosis regulator protein 1 and has been tested in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected CCAR1 in HeLa, A431, and HUVEC cells. Western Blotting Analysis: 0.5 µg/mL from a representative lot detected CCAR1 in 10 µg of HepG2 cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fragment corresponding to 173 amino acids from the N-terminal half of human Cell division cycle and apoptosis regulator protein 1 (CCAR1). |
| Clone | 4B9.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4B9.1 detects Cell division cycle and apoptosis regulator protein 1 (CCAR1) in human cells. It targets an epitope witihin 173 amino acids from the N-terminal half. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~135 kDa observed; 132.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected CCAR1 in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |