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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1526 |
| Description | Anti-ZCCHC11 (TUT4) Antibody, clone 9F6.1 |
| Alternate Names |
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| Background Information | Terminal uridylyltransferase 4 (EC 2.7.7.52; UniProt Q5TAX3; also known as TUTase 4, Zinc finger CCHC domain-containing protein 11) is encoded by the ZCCHC11 (also known as KIAA0191, TUT4) gene (Gene ID 23318) in human. TUTase 4 is an uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. It is involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets and also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, and miR-143 and miR-200c. It is reported to catalyze the 3 uridylation of miR-26A that targets IL6 transcript to abrogate the silencing of IL6 transcript and promote cytokine expression. It may also participate in toll-receptor signaling by suppressing TRAF6-dependent activation of NF-kappaB. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ZCCHC11 (TUT4), clone 9F6.1, Cat. No. MABE1526, is a highly specific mouse monoclonal antibody that targets ZCCHC11 (TUT4) and has been tested in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 4 µg/mL from a representative lot detected ZCCHC11 (TUT4) in 10 µg of C2C12 mouse myoblast and Jurkat human T-cell lysates. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human ZCCHC11 (TUT4) N-terminal fragment. |
| Clone | 9F6.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 9F6.1 targets an epitope in the N-terminal region of ZCCHC11 (TUT4). |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~185 kDa observed. 185.2/81.14 kDa (human isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in PC3 cell lysate. Western Blotting Analysis: 4 µg/mL of this antibody detected ZCCHC11 (TUT4) in 10 µg of PC3 human prostate cancer cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |