Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IHC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1041 |
| Description | Anti-Pax-9 Antibody, clone 7C2 (P9C23) |
| Alternate Names |
|
| Background Information | Paired box protein Pax-9 (UniProt P47242; also known as Paired box gene 9) is encoded by the Pax9 gene (Gene ID 18511) in murine species. Pax9 belongs to a family of nine transcription factors (Pax1-9) characterized by the presence of a DNA-binding ‘paired’-domain. Pax family transcription factors regulate the morphogenesis of a wide range of organs and are key factors for the development of mammalian sensory organs such as the eye (Pax6, Pax2), nose (Pax6), ears (Pax2, Pax8), and the endoderm-derived taste papillae (Pax9). In addition, Pax9 expression in the normal epithelium of the adult human oesophagus is reported to be either lost or significantly reduced in the majority of invasive carcinomas and epithelial dysplasias (precancerous lesions). The drop in the percentage of Pax9-positive cells in the epithelium decreases with increasing malignancy of the epithelial lesion. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Pax-9 Antibody, clone 7C2 (P9C23), Cat. No. MABE1041, is a highly specific rat monoclonal antibody, that targets Pax9 and has been tested in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Pax-9 in 10 µg of HepG2 cell lysate. Western Blotting Analysis: A representative lot detected Pax-9 in mouse and human oesophagus tissue lysate (Gerber, J.K., et al. (2002). J Pathol. 197(3):293-297). Immunohistochemistry Analysis: A representative lot detected Pax-9 in mouse and human oesophagus tissue sections (Gerber, J.K., et al. (2002). J Pathol. 197(3):293-297). |
| Biological Information | |
|---|---|
| Immunogen | MBP-tagged recombinant protein corresponding to the C-terminal region of mouse Pax-9. |
| Epitope | C-terminal region |
| Clone | 7C2 (P9C23) |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~36 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MOLT 4 cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected Pax-9 in 10 µg of MOLT 4 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |