Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC967 |
| Description | Anti-Kallikrein 7/KLK7 Antibody, clone 73-1 |
| Alternate Names |
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| Background Information | Kallikrein-7 (UniProt: P49862; also known as hK7, Serine protease 6, Stratum corneum chymotryptic enzyme, hSCCE) is encoded by the KLK7 (also known as PRSS6, SCCE) gene (Gene ID: 5650) in human. KLK7 is a member of the 15-member kallikrein subfamily of serine proteases. Human KLK7 displays 40 60% amino acid homology with other members of the family. Members of this family are involved in diverse physiological functions and serve as biomarkers for cancer. Increased expression of KLK7 is associated with unfavorable prognosis and progression of ovarian cancer. Less differentiated tumors of more advanced stage show higher level KLK7 expression. KLK7 has chymotrypsin-like activity and cleaves proteins with aromatic side chains in the P1 position. Its activity is inhibited by Zn2+ and Cu2+. KLK7 catalyzes the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. It is also reported to play a role in the activation of precursors to inflammatory cytokines. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Sodium bicarbonate buffer, pH 8.2 with 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect KLK7 using this mouse monoclonal Anti-Kallikrein 7/KLK7 Antibody, clone 73-1, Cat. No. MABC967. It was used in ELISA and Immunohistochemistry (Paraffin). |
| Key Applications |
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| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Kallikrein 7/KLK7 in human kidney tissue and human bone marrow. ELISA Analysis: A representative lot detected Kallikrein 7/KLK7 in ELISA applications (Shan, S.J., et. al. (2006). Clin Chem. 52(10):1879-86; Kishi, T., et. al. (2004). Clin Chem. 50(4):709-16). |
| Biological Information | |
|---|---|
| Immunogen | A full length recombinant human pro-Kallikrein 7. |
| Clone | 73-1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 73-1 specifically detects kallikrein 7 (KLK7) in human. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| Molecular Weight | 27.53 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human skin tissue. Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Kallikrein 7/KLK7 in human skin tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |