Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Mk | WB, ICC, IH(P) | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC953 |
| Description | Anti-Prohibitin-2/PHB2 Antibody, clone 7F8E3 |
| Alternate Names |
|
| Background Information | Prohibitin-2 (UniProt Q99623; also known as B-cell associated protein, B-cell receptor-associated protein BAP37, BCAP37, D-prohibitin, PHB2, Repressor of estrogen receptor activity) is encoded by the PHB2 (also known as BAP, REA) gene (Gene ID 11331) in human. Prohibitins are assembled into a ring-like PHB complex with 16-20 alternating Phb1 and Phb2 subunits in the inner mitochondrial membrane, where they function as chaperone for respiration chain proteins or as important structural scaffold for optimal mitochondrial morphology and function. Prohibitins are also reported to modulate transcriptions by interacting with various transcription factors, including nuclear receptors. Upregulated expression of prohibitins, septin 9, neurabin 1, and other cytoskeletal and functional proteins is found at the glutathione S-transferase placental-form-positive (GST-P+) hepatocellular carcinoma (HCC) foci during initiation and/or promotion stages of rat hepatocarcinogenesis, implicating an involvement of prohibitins in the neoplastic transformation of rat liver preneoplastic lesions. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Prohibitin-2/PHB2 Antibody, clone 7F8E3 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin) for the detection of Prohibitin-2/PHB2. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Prohibitin-2/PHB2 in 10 µg of COS-7 cell lysate. Immunocytochemistry Analysis: 4 µg/mL from a representative lot detected Prohibitin-2/PHB2 in A431 and HUVEC cells. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Prohibitin-2/PHB2 in human breast and mouse liver tissue. Immunohistochemistry Analysis: A representative lot detected upregulated Prohibitin-2 immunoreactivity at the glutathione S-transferase placental-form–positive (GST-P+) hepatocellular carcinoma (HCC) foci in frozen liver sections from rats subjected to hepatocarcinogenesis induction by N-nitrosodiethylamine (DEN) and Phenobarbital (PB) treatment (Kakehashi, A., et al. (2011). Toxicol Sci. 119(1):61-72). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Prohibitin-2/PHB2. |
| Epitope | Near C-terminus |
| Clone | 7F8E3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Expected to react with both spliced isoforms. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~34 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat C6 glioma cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Prohibitin-2/PHB2 in 10 µg of rat C6 glioma cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |