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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC946 |
| Description | Anti-PKCζ Antibody, clone 14B12.3 |
| Alternate Names |
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| Background Information | Protein kinase C zeta type (EC 2.7.11.13; UniProt Q05513; also known as nPKC-zeta, PKC-zeta) is encoded by the PRKCZ (also known as PKC2) gene (Gene ID 5590) in human. Protein kinase Cs (PKCs) are threonine/serine kinases that play important roles in cellular signal transductions. PKC isozymes are classified into three major classes based on their domain compositions and activator requirements. The conventional PKCs (cPKCs) include the alpha, beta I, beta II, and gamma isoforms. The novel PKCs (nPKCs) include the delta, epsilon, eta, and theta isoforms. Atypical PKCs (aPKCs) include the lambda (mouse ortholog to human PKC-lambda is called PKC-iota) and zeta isoforms. Both cPKCs and nPKCs bind diacylglycerol (DAG) via their C1 domain, while only cPKCs, but not nPKCs bind Ca+2 via their C2 domain. The aPKCs do not bind either DAG or calcium for activity. All three classes of PKCs contain a pseudosubstrate sequence in their N-terminal region that keeps the substrate-binding cavity occupied via intramolecular interaction when the enzymes are in their inactive conformation. Conformation change upon enzyme activation frees the substrate-binding site. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PKCζ Antibody, clone 14B12.3 is an antibody against PKCζ for use in Western Blotting, Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PRKCZ in 10 µg of PC12 cell lysate. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PRKCZ in human cerebral cortex, human cerebellum and mouse brain tissues. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human PKCζ C-terminal fragment. |
| Epitope | C-terminal region |
| Clone | 4B12.3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with all three spliced isoforms of human PKC-zeta. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~70 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected PKCζ in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |