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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC663 |
| Description | Anti-TICAM1 Antibody, clone 3D5.1 |
| Alternate Names |
|
| Background Information | TIR domain-containing adapter molecule 1 (UniProt Q8IUC6), also known as MyD88-3, Proline-rich, vinculin and TIR domain-containing protein B, Putative NF-kB-activating protein 502H, TICAM-1, TIR domain containing adaptor inducing interferon-b, TIR domain-containing adapter protein inducing IFN-b, Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon b) is encoded by the TICAM1 gene in human. TCAM-1/TRIF/MyD88-3 is as an adaptor molecule that mediates Toll-like receptor 3 (TLR3)-dependent NF-kB activation and cytokine expression. Upon recognition of double-stranded RNA, TLR3 recruits TRIF, which in turn associates with TNF receptor-associated factor 3 (TRAF3) to activate two Ik-B kinase (IKK)-related kinases, TANK-binding kinase 1 (TBK1) and IKKi. These two kinases phosphorylate interferon regulatory factor 3/7 (IRF3/7), resulting in the induction of type I interferons (IFNs) and expression of IFN-inducible genes. TICAM-1 can also mediate NF-kB activation through receptor-interacting protein 1 (RIP1) and TRAF6. Human enterovirus 68 (EV68) is reported to disable TLR3 pathway signaling by cleaving TRIF in infected cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TICAM1 Antibody, clone 3D5.1 is an antibody against TICAM1 for use in Western Blotting, Immunohistochemistry, Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TICAM1 in human liver tissue. Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected TICAM1 in HeLa and A431 cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human TICAM1. |
| Clone | 3D5.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~76 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Raji cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected TICAM1 in 10 µg of Raji cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |