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Millipore This Anti-Mgcracgap/Cyk4 Antibody, Clone 5g5 Is Validated For Use In Western Blotting And Immunocytochemistry And Immunoprecipitation For The Detection Of Mgcracgap/Cyk4. - Mill (Additional S&H or Hazmat Fees May Apply)
MgcRacGAP/CYK4 is a cytoskeletal interacting protein that participates in cellular functions from cell division and differentiation to ion transport, spermatogenesis and protein transport. MgcRacGAP/CYK4 is a component of the multi-protein complex called the centralspindlin complex that is key to cytokinesis function. MgcRacGAP/CYK4 developmentally is key for embryogenesis, adipocyte and myoblast development. It is also key in male germ development. MgcRacGAP/CYK4 associates with various proteins including alpha, beta and gamma tubulin, RACGAP1, and KIF23. MgcRacGAP/CYK4 can be localized in the nucleus but also in the cytoplasm where it is associated with the cytoskeletal spindle and microtubules. MgcRacGAP/CYK4 is highly expressed in testis, thymus and placenta but also expressed in spleen and various lymphocytes and sperm.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing PBS without preservatives with 50% glycerol.
Quality Level
MQ100
Applications
Application
This Anti-MgcRacGAP/CYK4 Antibody, clone 5G5 is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of MgcRacGAP/CYK4.
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
Application Notes
Immunocytochemistry Analysis: A representative lot of this antibody detected MgcRacGAP/CYK4 in HeLa cells (Uehara, R., et al. (2013) JCB. 202(4):623-636).
Immunoprecipitation Analysis: A representative lot of this antibody detected MgcRacGAP/CYK4 by immunoprecipitation.
Biological Information
Immunogen
Linear peptide corresponding to Human MgcRacGAP/CYK4.
Clone
5G5
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001119575
Gene Symbol
RACGAP1
MGCRACGAP
Purification Method
Protein A Purfied
UniProt Number
Q9H0H5
Molecular Weight
~75 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa nuclear extract cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected MgcRacGAP/CYK4 in 10 µg of HeLa nuclear extract cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
