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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, IP, IF | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC40 |
| Description | Anti-LAMP-2 Antibody, clone ABL-93 |
| Alternate Names |
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| Background Information | Lysosome-associated membrane protein 2 (LAMP-2) is a highly glycosolated protein belonging to the lysosome-associated membrane protein family. This group pf proteins composes a significant portion of the lysosomal membrane and are considered crucial mediators for cellular adhesion by interacting with carbohydrate receptors. LAMP-2 is a profuse and critical component of the lysosomal membrane as it has been implicated in playing a role in lysosomal biogenesis. It also appears to be involved in later stages of phagocytosis and autophagy, where it may act to protect the lysosomal membrane from being digested, assist in signal transduction, moderate levels of acidity within the lysosomal environment, and participate in adhesion. Mutations in LAMP-2 expression have been associated with lysosomal storage diseases or LSDs, along with Danon disease, an X-linked disorder characterized by skeletal myopathy, mental retardation, and hypertrophic cardiomyopathy. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LAMP-2 Antibody, clone ABL-93 is an antibody against LAMP-2 for use in WB, IP & IF. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: This antibody has been shown to immunoprecipitate LAMP-2 in HaNIH cells, (Chen, 1985). Immunofluorescence Analysis: This antibody has been shown to detect LAMP-2 in mouse brain, (Ferguson, 2009). |
| Biological Information | |
|---|---|
| Immunogen | Purified glycoproteins from NIH/3T3 cells, corresponding to mouse LAMP-2. |
| Clone | ABL-93 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | This antibody recognizes LAMP-2. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G |
| UniProt Number |
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| UniProt Summary | FUNCTION: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane. PTM: O- and N-glycosylated; some of the N-linked glycans are polylactosaminoglycans (by similarity). SEQUENCE SIMILARITIES: Belongs to the LAMP family. |
| Molecular Weight | ~ 80 kDa observed MW. The calculated MW is 45 kDa. LAMP-2 is heavily glycosoated and has been routinely observed at MWs of 80-120 kDa based on publication data, (Chen, 1985; Ferguson, 2009). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-2 in 10 µg of NIH/3T3 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |