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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P), FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC34 |
| Replaces | MABN16 |
| Description | Anti-Beclin-1 Antibody, clone 9A1.1 |
| Alternate Names |
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| Background Information | The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinsons and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Beclin-1 Antibody, clone 9A1.1 detects level of Beclin-1 & has been published & validated for use in Western Blotting, IHC(P), Flow Cytometry. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Beclin-1 in human colorectal adenocarcinoma tissue. Flow Cytometry Analysis: 1 µg from a representative lot detected Beclin-1 in HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human Beclin-1. |
| Clone | 9A1.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~54 kDa kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Raji cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Beclin-1 in 10 µg of Raji cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |