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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC272 |
| Description | Anti-Annexin VI Antibody, clone 4B11.1 |
| Alternate Names |
|
| Background Information | The protein Annexin A6 or alternatively known as 67 kDa calelectrin, Annexin VI, Annexin-6, Calphobindin-II (CPB-II), Chromobindin-20, Lipocortin VI, Protein III, p68, or p70 and encoded by the human gene named ANXA6 or ANX6. Annexin VI is part of a large family of Annexin proteins and shares with the family the ability to bind negatively charged phospholipids in a calcium dependent manner. Localized in the plasma membrane annexins are important in many cellular functions as the provide scaffolds and play roles in vesicle transport, exocytosis, endocytosis and calcium transport and conduction. Recent research indicates that Annexin VI can interact with PKCalpha and EGFR in A431 cells to help regulate the phosphorylation of pY-EGFR and the associated activation of downstream signaling events associated with cancer. Annexin VI in normal tissue is expressed most strongly in myocardial tissue where it associates with sarcomeric α-actinin and is part of the sarcomere structure. Annexin VI also regulates terminal chondrocyte differentiation by modulating key signaling pathway activities. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Annexin VI Antibody, clone 4B11.1 is validated for use in western blotting & ICC for the detection of Annexin VI. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1 µg/mL from a representative lot detected Annexin VI in 10 µg of C2C12 cell lysate. Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Annexin VI in HeLa and A431 cells. Alexa Fluor® is a registered trademark of Life Technologies®. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human Annexin VI. |
| Clone | 4B11.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~75 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Annexin VI in 10 µg of SH-SY5Y cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |