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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB88917 |
| Replaces | 04-1108 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Vitronectin Antibody, clone 8E6(LJ8) |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid. Buffer = 0.01M Phosphate buffer containing no preservative, pH 7.1. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Vitronectin Antibody, clone 8E6(LJ8) detects level of Vitronectin & has been published & validated for use in ELISA, WB, IH. |
| Key Applications |
|
| Application Notes | Western blot: 5-20 μg/mL Stains cell-binding vitronectin. Immunocytochemistry: 5-20 μg/mL ELISA: 5-20 μg/mL Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified human vitronectin from plasma. |
| Clone | 8E6(LJ8) |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The antibody reacts with human vitronectin, it does not react with human fibronectin (Hayman et al., 1983). |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P04004 # Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. SIZE: 478 amino acids; 54306 Da SUBUNIT: Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1 and insulin. SUBCELLULAR LOCATION: Secreted, extracellular space. TISSUE SPECIFICITY: Plasma. DOMAIN: SwissProt: P04004 The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. PTM: Sulfated on 2 tyrosine residues. & N- and O-glycosylated (By similarity). & Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. & It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.SIMILARITY: Contains 4 hemopexin-like domains. & Contains 1 SMB (somatomedin-B) domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 12 months.Avoid repeat freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |