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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H | IHC | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB5504A4 |
| Description | Anti-Vesicular Glutamate Transporter 2 Antibody, Alexa Fluor® 488 |
| Alternate Names |
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| Background Information | Vesicular Glutamate Transporter 2 (VGLUT2) is a sodium dependent inorganic phosphate cotransporter that is involved in the calcium dependent glutamate release from astrocytes. It is expressed in only a subset of neurons, localized to synaptic vesicles, at synapses exhibiting classical excitatory features. VGLUT2 mRNA is found in brain regions that lack VGLUT1, a related protein also displaying sodium dependent inorganic phosphate cotransporter activity. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Vesicular Glutamate Transporter 2 Antibody, Alexa Fluor 488 is validated for use in IH for the detection of Vesicular Glutamate Transporter 2. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein from rat VGLUT2. |
| Clone | 8G9.2 |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Mouse and Rat. Predicted to react with Human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Na+-dependent inorganic phosphate cotransporter; involved in Ca2+-dependent glutamate release from astrocytes [RGD]. |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas. May also mediate the transport of inorganic phosphate. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein (Potential). Cell junction, synapse, synaptosome. TISSUE SPECIFICITY: Expressed in brain (at protein level). Expressed in brainstem, deep nuclei, septal nuclei, nuclei of the diagonal band, frontal cortex, hypothalamus, midbrain, parietal cortex and temporal cortex. Also expressed in pineal gland and islets of Langerhans. SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. SIZE: 582 amino acids; 64392 Da |
| Molecular Weight | The unconjugated parent antibody (Cat. No. MAB5504) has an observed molecular weight at ~56 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in adult mouse brain tissue. Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Vesicular Glutamate Transporter 2 in adult mouse brain tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |